DifferexTM与Chelex-100法在混合斑检验中的应用比
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马骏;张良宾
【期刊名称】《中国法医学杂志》 【年(卷),期】2011(026)004
【摘要】目的 探讨DifferexTM系统法在法医学性侵犯案件混合斑检验中的应用价值,并与传统Chelex-100法进行比较.方法 对日常性侵犯案件中的生物检材分别通过DifferexTM系统法与Chelex-100法检验来比较两种方法的优劣.结果 采用Chelex-100法提取时,在所检验样本中仅有床单全部获得单一分型,而采用DifferexTM系统法则只有一条内裤的分型为混合型,其它均为单一分型.应用DiifferexTM系统法还可以同时提取女性上皮细胞DNA,成功率为100％.采用Chelex-100法时出现双尖峰的频率也明显超过DifferexTM系统法.结论 DifferexTM系统法在法医学性侵犯案件混合斑检验中比Chelex-100法具有更明显的优势,是一种非常有应用前景的方法.%Objective To evaluate the application value of the Differex? system in DNA extraction of mixture stains from sexsual assault cases,and compare it with Chelex-100 method. Methods DNA of mixture stain samples from sexsual assault cases was extracted by using the Differex? system and Chelex-100 method respectively, and then DNA typing results were compared. Results By using the Differex? system, the single typing was obtained from all samples except one pants. With the Chelex-100 method, however, the single typing was obtained only from the bed sheets.
DifferexTM与Chelex-100法在混合斑检验中的应用比较
