木质纤维素酶高产菌株的筛选及其对玉米秸秆降解效果的
影响
郭红伟;王娇;常娟;尹清强;左瑞雨;郑秋红;王平;王潇;刘俊熙
【期刊名称】《生物加工过程》 【年(卷),期】2012(010)005
【摘要】为了提高玉米秸秆中木质素的降解率,从腐烂的树枝和土壤中筛选木质纤维素酶高产菌株,以秸秆为唯一C源富集培养后,采用PDA -愈创木酚法进行初筛,筛选出产木质素酶真菌5株,然后以玉米秸秆为主要C源进行固态发酵和复筛.结果表明:第5号菌株在发酵玉米秸秆5d后,使木质素的降解率达到最高(34.95%),粗纤维的降解率达到20.00%,显著高于其他4种菌株(P<0.05),其羧甲基纤维素酶比酶活达到116.35 U/g;10 d后,其木质素酶比酶活达到最高(45.64U/g).%In order to increase corn stalk degradation, five strains of ligninase-producing microbes were selected from rotten plants and soil with the guaiacol as the indicator. The results showed that the fifth strain could produce CMCase at 116.35 U/g, degradation rates of lignin and crude fiber reached 34. 95% and 20. 00% (P <0. 05) after 5 d solid-state fermentation. The ligninase activity after 10 d fermentation medium reached 45. 64 U/g. 【总页数】5页(39-43)
【关键词】木质素酶;玉米秸秆;降解率
【作者】郭红伟;王娇;常娟;尹清强;左瑞雨;郑秋红;王平;王潇;刘俊熙
【作者单位】河南农业大学牧医工程学院,郑州450002;河南农业大学牧医工程