Culture and Differentiation of Human Orbital Preadipocytes in Thyroid-associated Ophthalmopathy

Culture and Differentiation of Human Orbital

Preadipocytes in Thyroid-associated

Ophthalmopathy

Lifei Wang;Zhongyao Wu;Jianhua Yan;Huasheng Yang;Zhicong Chen

【期刊名称】《眼科学报（英文版）》 【年(卷),期】2005(021)001

【摘要】Purpose: To identify whether there are preadipocytes in the orbits of thyroid-associated ophthalmopathy (TAO) patients and whether

they

possess

the

capacity

to

undergo

adipocytic

differentiation.Methods: Orbital fat tissue from TAO patients was placed in primary culture and proliferating cells were sub-passaged. The preadiocytes were identified with immunohistochemistry. Confluent preadipocytes were subjected to a differentiation protocol. And Peroxisome proliferators-associated receptorγ (PPARγ)transcript levels were determined before and after differentiation.Result: In primary culture, cells were spindly and showed positive immunostaining for preadipocyte factor-1 (pref-1 ) protein. During the process of differentiation, orbital cells showed evidence of adipogenesis and relatively greater PPARγ gene expression with differentiation.Conclusion: There are active preadipocytes in the orbital tissue of TAO patients which might be able to undergo adipocyte differentiation. These observations suggest that the preadipocytes differentiation contributed