HMGB -1在慢性肺栓塞患者血清中的表达及其与IL -1β、
TNF -α、D -二聚体的相关性研究
赵晓梅;闫巧焕;刘玲玲
【期刊名称】《现代中西医结合杂志》 【年(卷),期】2014(000)027
【摘要】目的：观测慢性肺栓塞（ CPE）患者血清中 HMGB -1的表达水平及其与 IL -1β、TNF -α、D -二聚体表达水平的相关性。方法 CPE 组选取 CPE 患者38例，对照组选取同期健康体检者42例。采用酶联免疫吸附（ ELISA）方法检测2组血清中 HMGB -1、IL -1β、TNF -α蛋白的表达水平，采用免疫比浊法测定血清中 D -二聚体表达水平，采用荧光定量 PCR 方法检测外周血单核细胞（PBMC）中 HMGB -1 mRNA 的表达水平。结果 CPE 组血清中 HMGB -1、IL -1β、TNF -α、D -二聚体的表达均明显高于对照组（P 均﹤0.05），且血清中 HMGB -1蛋白水平与 IL -1β、TNF -α、D -二聚体水平均呈正相关（ P 均﹤0.05)。CPE 组外周血单核细胞 HMGB -1 mRNA 表达量明显高于健康对照组( P ﹤0.05)。结论 HMGB%Objective It is to observe the expression of HMGB - 1 in patients with chronic pulmonary embolism( CPE) and its correlation with the expression of IL - 1β,TNF - α and D - dimer. Methods 38 patients of CPE were selected into CPE group and 42 healthy subjects in the same period in were selected into control group. The expressions of serum HMGB -1,IL - 1β,TNF - αwere detected by ELISA method. D - Dimer was detected by immunoturbidimetry. HMGB - 1 mRNA ex-pression was detected by
HMGB -1在慢性肺栓塞患者血清中的表达及其与IL -1β、TNF -α、D -二聚体
