Induction of antiproliferative effect by diosgenin through activation of p53,release of ap

Induction of antiproliferative effect by diosgenin through activation of p53,release of apoptosis-inducing factor (AIF) and modulation of caspase-3

activity in different human cancer cells

Cecile CORBIERE;Bertrand LIAGRE;Faraj TERRO;Jean-Louis BENEYTOUT

【期刊名称】《细胞研究（英文版）》 【年(卷),期】2004(014)003

【摘要】Previously, we demonstrated that a plant steroid, diosgenin, altered cell cycle distribution and induced apoptosis in the human osteosarcoma 1547 cell line. The objective of this study was to investigate if the antiproliferative effect of diosgenin was similar for different human cancer cell lines such as laryngocarcinoma HEp-2 and melanoma M4Beu cells. Moreover, this work essentially focused on the mitochondrial pathway. We found that diosgenin had an important and similar antiproliferative effect on different types of cancer cells. In addition, our new results show that diosgenininduced apoptosis is caspase-3 dependent with a fall of mitochondrial membrane potential, nuclear localization of AIF and poly (ADP-ribose) polymerase cleavage. Diosgenin treatment also induces p53 activation and cell cycle arrest in the different cell lines studied. 【总页数】9页(188-196)

【关键词】diosgenin, apoptosis, p53, AIF, caspase, cancer cells