金黄地鼠颊黏膜鳞癌动物模型的建立及其生物学特性的研
究
吕长坤;万澎波;马菲菲;罗春丽;万雪莲;王长海
【期刊名称】《重庆医学》 【年(卷),期】2013(000)021
【摘要】目的建立金黄地鼠颊黏膜鳞癌动物模型，观察其生物学特性。方法40只采用0．05％4-硝基喹啉-1-氧化物（4NQO）涂抹颊黏膜，10只采用自来水涂抹颊黏膜。苏木精-伊红（HE）、转录因子蛋白（Foxm1）免疫组织化学观察第8、12周组织标本。有限稀释法体外传代培养颊黏膜组织标本，观察平板克隆形成率，检测单克隆细胞内细胞角蛋白（C K ）和波形蛋白（Vim）表达，染色体分析确定细胞核型。结果第12周时26（84．6％）只颊黏膜病理为原位癌，HE染色、Foxm1免疫组织化学结果表明，符合鳞癌细胞基本特征，CK和Vim免疫组织化学阳性率约为96．0％，染色体为四倍体核型。结论4NQO涂抹诱发金黄地鼠颊黏膜癌变，建立金黄地鼠颊黏膜鳞癌动物模型。%Objective To establish the golden hamster model of buccal squamous
carcinoma
and
observe
its
biological
character-
istics .Methods 50 golden hamster were randomly divided into two group :experiment group (n=40) and control group(n=10) . Buccal mucosa of golden hamster were daubed by exposure 4-nitroquinoline-1-oxide (4NQO) in experiment group and tap water in control group .HE staining and immunohistochemistry were used to observe the tissue sample on the 8th and 12th week .The tissue samples of golden
hamster buccal-mucosa cancer were used for the in-vitro
subculture .Then flat cloning formation rate ,expression of CK and Vim ,and cell karyotype were detected .Results The observations of cell morphology and biology showed that the tissue of buccal squamous carcinoma were conformed to the basic characteristics of squamous carcinoma cell in 26 golden hamster(84 .6% ) after 12 weeks .The positive rate of CK and Vim were 96 .0% by immunohistochemical staining .The Chromosomes were tetraploid karyotype .Conclusion We successfully established the golden hamster model of buccal squamous carcinoma by the daub method of 4NQO . 【总页数】4页(2493-2495,2499)
【关键词】癌, 鳞状细胞;模型, 动物;金黄地鼠;颊黏膜 【作者】吕长坤;万澎波;马菲菲;罗春丽;万雪莲;王长海
【作者单位】重庆医科大学检验医学院 400016;商丘医学高等专科学校教务处，河南商丘476100;商丘医学高等专科学校教务处，河南商丘476100;重庆医科大学检验医学院 400016;商丘医学高等专科学校教务处，河南商丘476100;商丘医学高等专科学校教务处，河南商丘476100 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chongqing-medicine_thesis/0201231379670.html
【相关文献】
1.可移植性金黄地鼠颊肉瘤模型的建立及其生物学特性 [J], 章魁华; 杜平 2.四种人癌术后转移动物模型的建立及其生物学特性研究 [C], 徐冰; 姚明; 顾为望; 闫明霞; 刘蕾; 张杰
3.兔VX2直肠癌动物模型的建立及其生物学特性的观察 [C], 梁新梅; 程英升; 周碧
4.气虚血瘀证卵巢癌动物模型的建立及其生物学特性 [C], 齐聪; 张勤华
5.VX2移植性兔直肠癌淋巴结转移动物模型的建立及其生物学特性 [J], 孙轶群; 童彤; 毛健; 钟芳芳; 顾雅佳
以上内容为文献基本信息，获取文献全文请下载
金黄地鼠颊黏膜鳞癌动物模型的建立及其生物学特性的研究
