Reversible changes in aqueous outflow facility, hydrodynamics,and morphology following acu

Reversible changes in aqueous outflow facility, hydrodynamics,and morphology following acute intraocular pressure variation in bovine eyes

ZHU Jing-ying;YE Wen;WANG Ti;GONG Hai-yan

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2013(126)008

【摘要】Background Elevated intraocular pressure (lOP) is primarily due to increased aqueous outflow resistance,but how aqueous outflow resistance is generated and regulated are still not fully understood.The aim of this study is to determine whether changes in outflow facility,outflow pattern,and morphology following acute lOP elevation were reversible when the lOP was returned to a normal level in bovine eyes using a two-color tracer technique to label outflow patterns within the same eye.Methods Twelve fresh enucleated bovine eyes were perfused with Dulbecco's phosphate buffer saline (PBS) containing 5.5 mmol/L glucose (DBG) at 30 mmHg first to establish the baseline outflow facility followed by a fixed volume of red fluorescent microspheres (0.5 μm,0.002％ v/v).After the red tracer being replaced with DBG in the anterior chamber,perfusion was continued at 7 mmHg with the same volume of green tracer,followed by a fixative.In two control groups,the eyes were constantly perfused at either 30 mmHg (n=6) or 7 mmHg (n=6) using the same methods.The outflow facility