Effect of ions on the activity of brain acetylcholinesterase from tropical ifsh

Effect of ions on the activity of brain acetylcholinesterase from tropical ifsh

Caio Rodrigo Dias Assis;Amanda Guedes Linhares;Vagne Melo Oliveira;Renata Cristina Penha Frana;Juliana Ferreira Santos;Ranilson Souza Bezerra;Luiz Bezerra Carvalho Jr

【期刊名称】《海岸生命医学杂志（英文版）》 【年(卷),期】2015(000)007

【摘要】Objective:To investigate the effect of ions on brain acetylcholinesterase (AChE; EC 3.1.1.7) activities from economic important

fish

[pirarucu,Arapaima

gigas;

tambaqui,Colossoma

macropomum; cobia,Rachycentron canadum (R. canadum) and Nile tilapia,Oreochromis niloticus(O. niloticus)] comparing with a commercial enzyme from electric eel [Electrophorus electricus(E. electricus)]. Methods: Thein vitro exposure was performed at concentrations ranging from 0.001 to 10 mmol/L (except for ethylene diamine tetraacetic acid; up to 150 mmol/L). Inhibition kinetics onR. canadum andO. niloticus were also observed through four methods (Michaelis-Menten, Lineweaver-Burk, Dixon and Cornish-Bowden plots) in order to investigate the type of inhibition produced by some ions. Results: Hg2+, As3+, Cu2+, Zn2+, Cd2+ caused inhibition in all the species under study. Ca2+, Mg2+ and Mn2+ induced slight activation inR. canadum enzyme while Pb2+, Ba2+, Fe2+, Li+ inhibited the AChE from some of the analyzed species. The lowest IC50 and Ki values were estimated forE.