Cloning and Characterization of a Heterogeneous Nuclear Ribonucleoprotein Homolog from Pea

Cloning and Characterization of a Heterogeneous Nuclear Ribonucleoprotein Homolog from Pearl

Oyster, Pinctada fucata

Xunhao XIONG;Qiaoli FENG;Liping XIE;Rongqing ZHANG

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2007(039)012

【摘要】Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, a cDNA encoding a presumed full-length RNA-binding protein was isolated from pearl oyster (Pinctadafucata) using reverse transcription-polymerase chain reaction with degenerate primers, and rapid amplification of cDNA ends. The full-length cDNA consists of 2737 bp with an open reading frame encoding a protein of 624 amino acids with a Predicted molecular weight of 69 kDa and isoelectric point of 8.7. The putative pearl oyster RNA-binding protein presents a molecular organization close to the hnRNPs, namely an acidic N-terminal followed by three RNA-recognition motifs and a Cterminal that contains RG/RGG repeated motifs. When transfected HeLa cells, the Pf-HRPH (Pinctada fucata hnRNP homolog) gene expression product was found only in nuclei, revealing that it is a nuclear protein. The expression pattern was also investigated by quantitative real-time polymerase chain reaction,indicating that Pf-HRPH mRNA was abundantly expressed in

gonad, gill, and viscera. As far as we know,the putative Pf-HRPH is the first hnRNP homolog cloned in mollusks. These data are significant for further study of the multiple functions of RNA-binding protein. 【总页数】9页(955-963)

【关键词】RNA-recognition

motif;

heterogeneous

nuclear

ribonucleoprotein; glycine-rich protein;Pinctada fucata

【作者】Xunhao XIONG;Qiaoli FENG;Liping XIE;Rongqing ZHANG

【作者单位】Institute of Marine Biotechnology,Department of Biological Science

and

Biotechnology,Tsinghua of

Marine

University,Beijing

of

100084,China;Institute Biotechnology,Department

Biological Science and Biotechnology,Tsinghua University,Beijing 100084,China;Institute

of

Marine

Biotechnology,Department

of

Biological Science and Biotechnology,Tsinghua University,Beijing 100084,China;Protein

Science

Laboratory

of

the

Ministry

of

Education,Tsinghua University,Beijing 100084,China;Institute of Marine Biotechnology,Department

of

Biological

Science

and

Biotechnology,Tsinghua University,Beijing 100084,China;Protein Science Laboratory of the Ministry of Education,Tsinghua University,Beijing 100084,China 【正文语种】中文 【中图分类】Q5 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_acta-biochimica-biophysica-sinica_thesis/0201254943926.html 【相关文献】

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