猪原始生殖细胞的分离、培养与鉴定
李世杰;郑瑞珍;岳奎忠;陈瑛;杨增明
【期刊名称】《动物学报（英文版）》 【年(卷),期】2003(049)004
【摘要】Embryonic germ cells (EG cells) are pluripotential undifferentiated stem cells isolated from cultured primordial germ cells (PGCs). Like ES cells, EG cells are of importance for gene targeting, therapeutical cloning and organ trans-plantation. The aim of this study was to isolate and characterize EG cells from porcine PGCs. The genital ridges from 24- 26 days old porcine embryos were treated in 0.02% EDTA for 20 min and pricked with a needle to release PGCs. The isolated PGCs were cultured on a SNL feeder layer in an EG cell medium. The EG cell medium consisted of Dulbecco''s modified Eagle'' s medium (DMEM) supplemented with 20 % Buffalo rat lever (BRL) cell-conditioned medium, 15 % fe-tal bovine serum, 1 mmol L-glutamine, 0.1 mol nonessential
amino
acids,
10
μmol
β-mercaptoethanol
and
antibiotics.The freshly isolated PGCs were positive for alkaline phosphatase activity and Periodic acid-Schiff'' s staining. Under this culture regime, PGCs could be maintained in an undifferentiated state and used for further cultures. One strain of the cul-tured PGCs was cultured 8 times, and alkaline phosphatase activity was detected in the colony
formed
from
this
strain.These
cultured
PGCs
could
spontaneously differentiate into fibroblast-like cells. These data suggested that we had success-fully isolated EG-like cells from oorcine PGCs.
【总页数】5页(529-533)
【关键词】猪原始生殖细胞;胚胎;胚胎干细胞 【作者】李世杰;郑瑞珍;岳奎忠;陈瑛;杨增明
【作者单位】东北农业大学生命科学学院,哈尔滨,150030;中国科学院遗传与发育生物学研究所,北京,100080;东北农业大学生命科学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030 【正文语种】中文 【中图分类】Q95 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_current-zoology_thesis/0201254537005.html 【相关文献】
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猪原始生殖细胞的分离、培养与鉴定
