C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin

C-terminus of TRAP in Staphylococcus can enhance

the activity of lysozyme and lysostaphin

Guang Yang;Ningsheng Shao;Yaping Gao;Jiannan Feng;Yong Huang;Shaohua Li;Yu Liu;Chuan Liu;Ming Fan;Beifen Shen

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2008(040)005

【摘要】In Staphylococcus aureus, the target of RNAⅢ activating protein (TRAP) is a membrane-associated protein whose Cterminus can be used as a vaccine to provide protection against staphylococcal infection. Here, we show for the first time by surface plasmon resonance and enzyme-linked immunosorbent assay that TRAP can specifically bind lysozyme and iysostaphin through its C-terminus (amino acids 155-167) and enhance lysozomal activities in vitro. It was also found that the traP mutant strain is more resistant to iysostaphin than wild-type. Our previous data showed that the C-terminus of TRAP might be extracellular. So our results suggested that the C-terminus of TRAP could act as the specific targeting protein of the lysozyme/lysostaphin on the S. aureus cell wall and the biological significance of the interaction might be to facilitate lysozyme/lysostaphin-mediated cell iysis.

【总页数】7页(452-458)

【关键词】Staphylococcus aureus; TRAP; lysozyme;lysostaphin

【作者】Guang Yang;Ningsheng Shao;Yaping Gao;Jiannan Feng;Yong Huang;Shaohua Li;Yu Liu;Chuan Liu;Ming Fan;Beifen Shen

【作者单位】Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Department of Chemistry, Peking University, Beijing 100086, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Beijing Institute of Basic Medical Sciences, Beijing 100850, China 【正文语种】中文 【中图分类】Q5 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_acta-biochimica-biophysica-sinica_thesis/0201254943192.html 【相关文献】

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