毒胡萝卜素对转化生长因子β1刺激的小鼠肺成纤维细胞凋
亡的影响
闫宇;马思琪;肖倩;曲银娥;肖永红
【期刊名称】《中国临床药理学杂志》 【年(卷),期】2017(033)019
【摘要】Objective To explore the effects of thapsigargin on the cell cycle,apoptosis and cysteinyl aspartate specific proteinase (Caspase-12) protein expression in mouse lung fibroblast stimulated transforming growth factor-β1 (TGF-β1).Methods Mice lung fibroblast lines frozen in liquid nitrogen were recovered and cultured.Experiment was divided into three groups:the control group,model group and experimental group.RPMI-1640 medium was only add in the control group,5 ng · mL-1 TGF-β1 was add in the model group;while in experimental group,5 ng · mL-1 TGF-β1 was add for 24 hours,then 4 pmol · L-1 thapsigargin was add.After 24 h,the cell cycle and apoptosis were detected by flow cytometry instrument.The protein expression of caspase-12 was examined using the immunohistochemical method.Results In control group,model group and experimental group,the proportion of G1 phase cells were (58.72 ±8.35)％,(52.13 ±7.61)％ and (73.02 ±9.32)％,which of S phase cells were (29.50 ± 4.24) ％,(40.54 ± 6.31) ％ and (21.66 ± 4.17) ％,G2 phase cells was (11.75 ± 0.96) ％,(7.62 ± 3.22)％ and (6.09 ± 2.11)％ respectively,the differences had statistical significance in G1
毒胡萝卜素对转化生长因子β1刺激的小鼠肺成纤维细胞凋亡的影响
