人参皂苷Rg3对乳腺癌MCF-7细胞增殖和侵袭的影响
陈云;张竝;俞勇;金冶
【期刊名称】《中国现代应用药学》 【年(卷),期】2013(030)007
【摘要】OBJECTIVE To investigate the effect of ginsenoside Rg3 on cell proliferation and invasion of estrogen receptor(ER) positive breast cancer MCF-7 cell line and the potential mechanism.METHODS The proliferation of MCF-7 cell was detected by MTT assay.Cell cycle and apoptosis rate were analyzed by flow cytometry.The effect on cell invasion was assayed by using the transwell methods.The expression of MMP-9 mRNA in MCF-7 cell was analyzed with the RT-PCR.RESULTS Compared with the control group,ginsenoside Rg3 could significantly inhibit the proliferation of MCF-7 breast cancer cells.The cell cycle of MCF-7 was also changed after treated with the ginsenoside Rg3.The percentage of cells in G0/G1 and S phase were decreased,whereas the percentage of cells in G2/M was increased significantly.ginsenoside Rg3 could also induce the MCF-7 cell apoptosis and decrease the index of cell
invasion
on
a
time
and
dose-depended
manner.Meanwhile,ginsenoside Rg3 could significantly suppress the expression of MMP-9 mRNA on MCF-7 cell(P＜0.05).CONCLUSION ginsenoside Rg3 could inhibit the proliferation and invasion of breast cancer MCF-7 cell line,the underlying mechanism may be related with
the inhibitory effect of ginsenoside Rg3 on MMP-9 mRNA expression in MCF-7 cell.%目的 观察人参皂苷Rg3对雌激素受体阳性的乳腺癌细胞MCF-7增殖和侵袭的影响,并探讨其可能的作用机制.方法 采用MTT法检测细胞的增殖能力,流式细胞仪分析细胞周期分布以及凋亡比率,通过Transwell小室观察细胞侵袭力,RT-PCR法检测细胞中的MMP-9 mRNA的表达.结果 与对照组相比,人参皂苷Rg3能显著抑制MCF-7细胞的增殖;G0/G1期及S期细胞比例减少,而G2/M期细胞比例显著增加;同时细胞凋亡比率亦明显提升,而细胞侵袭指数降低,且呈现良好的剂量、时间依赖性.同时人参皂苷Rg3还能显著抑制细胞中MMP-9 mRNA的表达水平(P＜0.05).结论 人参皂苷Rg3能抑制MCF-7细胞的增殖和侵袭,其作用机制可能与其能降低MMP-9基因的表达有关. 【总页数】4页(722-725)
【关键词】人参皂苷Rg3;乳腺癌;基质金属蛋白酶-9;细胞侵袭 【作者】陈云;张竝;俞勇;金冶
【作者单位】浙江省立同德医院,杭州310012;浙江省立同德医院,杭州310012;浙江省立同德医院,杭州310012;浙江省立同德医院,杭州310012 【正文语种】中文 【中图分类】R285.5 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-modern-applied-pharmacy_thesis/0201241999877.html 【相关文献】
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人参皂苷Rg3对乳腺癌MCF-7细胞增殖和侵袭的影响
