β淀粉样蛋白1～42促进U87细胞凋亡作用的研究

β淀粉样蛋白1～42促进U87细胞凋亡作用的研究

申茉函;王全才

【期刊名称】《中国老年学杂志》 【年(卷),期】2015(000)004

【摘要】Objective To discuss the mechanisms of recombinantβ-amyloid peptide 1~42(Aβ1~42 ) on U87 cell toxicity.Methods MTT assay,inverted microscope,transmission electronic microscopy and flow cytometry were used to detect the mechanisms of recombinant Aβ1~42 on U87 cell toxicity.Results Treated with Aβ1~42 on U87 cells after 24 h,Aβ1~42 caused U87 cells metabolic rate decreasing with dose dependence,the characteristics of the apoptosis cells were found by inverted microscope and transmission electronic microscopy.The U87 cell apoptosis rate in 10,20,50

μmol/L

Aβ1~42

groups

were

35.6%,42.2%,58.1%

respectively.Conclusions Aβ1~42 induces U87 cell damage mainly be the way of apoptosis.%目的：研究重组人β淀粉样蛋白（ Aβ）1～42对人神经胶质瘤细胞U87毒性作用机制。方法用MTT检测代谢率，倒置显微镜、投射电镜以及流式细胞术技术研究Aβ1～42对U87细胞的损伤作用机制。结果用Aβ1～42处理U87细胞24 h后，Aβ1～42剂量依赖性地引起U87细胞的MTT代谢率减少。倒置显微镜及透射电镜观察发现经Aβ1～42处理的 U87细胞表现出凋亡细胞的特征。流式细胞仪检测表明10、20、50μmol／L 的Aβ1～42组U87细胞的凋亡率分别为35．6％，42．2％，58．1％。结论 Aβ1～42致U87细胞发生损伤主要是通过细胞凋亡的途径。