麸炒白术配方颗粒特征图谱及白术内酯Ⅲ含量测定研究
黄妍;姚建标;赖云枫;王如伟
【期刊名称】《中国现代应用药学》 【年(卷),期】2017(034)009
【摘要】目的 建立麸炒白术饮片和麸炒白术配方颗粒中白术内酯Ⅲ的含量测定方法,并同时建立麸炒白术配方颗粒的HPLC特征图谱.方法 采用色谱柱为Welch Xtimate C18柱(250 mmx4.6 mm,5μm);流动相为乙腈-水溶液,梯度洗脱;体积流量1.0 mL.min-1;柱温30℃;检测波长224 nm;进样量10 μL.结果 建立了麸炒白术饮片及颗粒中白术内酯Ⅲ的含量测定方法,白术内酯Ⅲ在0.031 3～0.782 4 μg内与色谱峰面积呈良好的线性关系;麸炒白术饮片和麸炒白术配方颗粒平均加样回收率分别102.0％(RSD=1.88％),101.0％(RSD=1.02％);建立了麸炒白术配方颗粒的HPLC特征图谱,确立了8个特征峰.结论 该方法准确、可靠,为麸炒白术配方颗粒的工艺研究及质量控制提供了参考依据.%OBJECTIVE To establish an HPLC method for the determination of atractylenolide Ⅲ in bran-processed atractylodes and bran-processed Atractylodes Formula granule,and to establish the specific chromatogram of bran-processed
Atractylodes
Formula
granule.METHODS
The
chromatographic separation was performed on a Welch Xtimate C18 column (250 mm×4.6 mm,5 am).The mobile phase was a mixture of acetonitrile-water in gradient elution at a flow rate of 1.0 mL.min-1.The detection wavelength was 224 nm and the column temperature was set at 30 ℃.RESULTS,The linear range of atractylenolide Ⅲ in bran-
麸炒白术配方颗粒特征图谱及白术内酯Ⅲ含量测定研究
