An Integrated Study for the Utilization of Anthraquinone Compounds Extract “Heshouwu”

An Integrated Study for the Utilization of Anthraquinone Compounds Extract “Heshouwu” In vivo and their Comparative Metabolism in Liver

Microsomes Using UPLC-ESI-Q-TOF/MS~n

Sha Chen;Hong-Yu Ma;Zhe Deng;Jun Zhang;Jin-Tang Cheng;Chang Chen;An Liu;

【期刊名称】《世界中医药杂志：英文版》 【年(卷),期】2018(000)001

【摘要】Objective: Anthraquinone(AQ), a major bioactive component of the traditional Chinese medicine He ShouW u, has widespread applications in industry and medicine. The objective of the current study is to explore the differences in the bioavailability of anthraquinones in vivo and the metabolism in liver microsomes. Materials and Methods: In vivo, we used a reliable UPLC?ESI?Qq Q?MS/MS method to measure seven AQ compounds in the jugular vein plasma of rats following oral administration of He Shou Wu. Furthermore, in order to quantify the bioavailability of AQs in vivo and to further understand the metabolism of these compounds, we compared the in vitro metabolism of AQ in different species with respect to metabolic profiles, the enzymes involved, and catalytic efficiency using liver microsomes from human(HLM), mouse(MLM), rat(RLM), and beagle dog(DLM). Results: We identified two metabolic pathways, including the hydroxylation and glucuronidation of AQ, in the liver microsomes of humans and other