The ATP-binding Cassette Transporter OsABCG15 is Required for Anther Development and Polle

The ATP-binding Cassette Transporter OsABCG15 is

Required for Anther Development and Pollen

Fertility in Rice

Bai-Xiao Niu;Fu-Rong He;Ming He;Ding Ren;Le-Tian Chen;Yao-Guang Liu

【期刊名称】《植物学报（英文版）》 【年(卷),期】2013(055)008

【摘要】Plant male reproductive development is a complex biological process,but the underlying mechanism is not well understood.Here,we characterized a rice (Oryza sativa L.) male sterile mutant.Based on mapbased cloning and sequence analysis,we identified a 1,459-bp deletion in an adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene,OsABCG15,causing abnormal anthers and male sterility.Therefore,we named this mutant osabcg15.Expression analysis showed that OsABCG15 is expressed specifically in developmental anthers from stage 8 (meiosis Ⅱ stage) to stage 10 (late microspore stage).Two genes CYP704B2 and WDA1,involved in the biosynthesis of very-long-chain fatty acids for the establishment of the anther cuticle and pollen exine,were downregulated in osabcg15 mutant,suggesting that OsABCG15 may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther locules.Consistently,histological analysis showed that osabcg15 mutants developed obvious abnormality in postmeiotic