Cyclin E表达对于原代乳腺癌细胞侵袭性的影响
方仁杏
【期刊名称】《现代肿瘤医学》 【年(卷),期】2011(019)004
【摘要】Objective: To study the Correlation between expression of Cyclin E in breast cancer and invasion of primary breast cancer cells. Methods: 1cm3 of the breast cancer tissue was taken got from operation, prepareing breast cancer cells suspension of origin generation, and detecting the expression level of Cyclin E by immunofluorescent technique. These cancer cells of origin generation were detected for invasion capability. Results: The invasion test presented that the average numher of cells of origin generation with overexpression of Cyclin E infiltrating Transwell PC micropore film was 76. 32 ± 11. 40 per high power field, the average number of cells of origin generation with negative expressivion of Cyclin E was 21. 56 ± 13. 22 per high power field P ＜ 0. 05. Conclusion : The expression of Cyclin E is related with the invasion of origin generation cells in breast cancer, the invasion of overexpression of Cyclin E is more powerful than negative expression of Cyclin E in breast cancer.%目的:从细胞学角度探讨Cycli E表达水平与原代乳腺癌细胞侵袭之间的关系.方法:手术中无菌条件下切取乳腺癌组织,制备原代乳腺癌细胞悬液,运用Transwell小室进行原代乳腺癌细胞侵袭力实验;获取具有不同侵袭力的原代乳腺癌细胞,再用免疫荧光技术检测不
Cyclin E表达对于原代乳腺癌细胞侵袭性的影响
