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一种优良半矮秆水稻品种的分离与分子鉴定
作者：林建中, 杨远柱, 赵小英,唐冬英, 周波, 杜长青,刘选明 来源：《湖南大学学报·自然科学版》2010年第08期
摘要: 株1S是中国南方稻区杂交水稻育种广泛应用的优良籼型温敏雄性核不育系. 为了改良其农艺性状, 我们采用体细胞无性系诱变方法筛选半矮秆突变体. 本文报道了2个体细胞无性系突变体SV1S 和SV14S的表型和初步分子鉴定结果. 与亲本株1S相比, 突变体的高度降低, 基部节间长度缩短, 节间壁显著增厚, 但是上部的节间变化不明显. 更重要的是, 我们发现赤霉素生物合成途径中的关键酶基因GA20ox-2出现了约200 bp的缺失突变, 导致该基因在半矮秆突变体中的转录水平降低. 研究结果表明, 突变体SV1S和SV14S很可能是由于同一缺失突变导致赤霉素合成受阻, 从而部分导致了半矮秆性状的出现. 然而, 2个突变体苗期的高度相同, 以及糊粉层细胞-淀粉酶活性和赤霉素敏感性降低等性状与以前报道的sd-1突变体有所不同. 因此, 我们推测在SV突变体中可能还存在第二个突变位点. 该结果也进一步证实了体细胞无性系诱变在水稻育种中具有良好的应用前景。
关键词:GA20ox-2; 赤霉素; 限制性片段长度多态性(RFLP); 水稻(Oryza sativa L.); 半矮秆 中图分类号: 文献标识码:
Isolation and Molecular Characterization of Semi-dwarf Mutations in an Elite Rice Cultivar LIN Jianzhong1, YANG Yuanzhu2, ZHAO Xiaoying1, TANG Dongying1, ZHOU Bo1,DU Changqing1, LIU Xuanming1*
(1. College of Biological Sciences, State Key Laboratory of Chemo-Biosensing and
Chemometrics, Hunan Univ, Changsha, Hunan 410082, China; 2. Academy of Seed Industry of Hunan Yahua, Changsha, Hunan 410001, China)
Abstract: Zhu1S is an elite indica thermo-sensitive genic male-sterile breeding stock widely used in hybrid rice production in Southern China. To improve key agronomic traits of this elite cultivar, we used somatic mutagenesis method to screen for semi-dwarf mutations. Here we report phenotypic and preliminary molecular characterization of two somatic mutants named SV1S and SV14S. Comparing to the parent Zhu1S, these mutations reduced the plant statures, shortened and thickened the lower internodes significantly but the upper internodes slightly. Furthermore, we found an about 200bp deletion in the GA20ox-2 gene encoding the key GA metabolic enzyme and little transcription of this gene in both semi-dwarf mutant lines. These results indicate that SV1S and SV14S mutants are more likely impaired in gibberellin metabolism derived from the same mutation that is partially responsible for their semi-dwarf phenotype. Whereas, both mutants have similar height at the seedling stage and reduce slightly GA sensitivity of -amylase activity in aleurone cells, which is different from the sd-
一种优良半矮秆水稻品种的分离与分子鉴定
