Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm

Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm

EST Database

Bao-Long NIU;Zhi-Qi MENG;Yue-Zhi TAO;Shun-Lin LU;Hong-Biao WENG;Li-Hua HE;Wei-Feng SHEN

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2005(037)011

【摘要】We have identified Bombyx mori transformer-2 gene (Bmtra-2) cDNA by blasting the EST database of B. mori. It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis. By comparing corresponding ESTs to the Bmtra-2 DNA sequence,it was revealed that there were eight exons and seven introns, and all splice sites of exons/introns conformed to the GT/AG rule. Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing. Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction. All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif, showing striking organizational similarity to Drosophila TRA-2 proteins. 【总页数】9页(728-736)

【关键词】EST database;transformer-2 gene;Bmtra-2;alternative