成纤维细胞生长因子21调控小鼠肝脏和脂肪细胞的糖脂代
谢
李钶;李伶;杨刚毅
【期刊名称】《中华内分泌代谢杂志》 【年(卷),期】2010(026)008
【摘要】Objective To investigate the role played by fibroblast growth factor-21 (FGF-21) in glucoselipid metabolism in mice adipocyte and hepatocyte. Methods FGF-21 over-expressive vector and FGF-21-shRNA vector were transfected to Hepa1-6 hepatocyte and 3T3-L1 adipocyte, respectively. FGF-21 protein levels were evaluated by Western blotting, glucose uptake rate (GUR) was evaluated by radioimmunoassay. The mRNA expression of transcription factors was detected by real-time quantitative PCR. Results Transfection of pcDNAFGF21 significantly increased FGF-21 expression in both liver and fat cells (4.8 fold and 4.2 fold, both P<0.05). Moreover, FGF-21 expressions were down-regulated by 86. 3% and 77. 8% with pGenesil-FGF21,respectively (both P<0.05 ). The FGF-21 up-regulation markedly increased GUR and decreased intracellular triglycerides content in the 3T3-L1 adipocyte. Meanwhile, the up-regulation of glucose transporter-1, insulin receptor substrate-1 (IRS-1), PPARγ, adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL),and adipocyte fatty acid binding protein 2 ( ap2 ) mRNA expressions were also observed ( all P<0. 05 ). The reverse changes
happened in FGF-21 deficient adipocyte except IRS-1. In hepatocyte, FGF-21 has no effect on GUR.FGF-21 over-expression reduced PPARγ, 3-hydroxy-3-methylglutaryl-CoA
reductase
(HMGR),
and
phosphoenolpyruvate carboxykinase (PEPCK) mRNA expressions, and increased low-density lipoprotein receptor expression (P<0.05). Conclusions FGF-21 regulates glucose homeostasis and accommodates intracellular fat content in adipocyte. In hepatocyte, FGF-21 plays an important role in gluconeogenesis and cholesterol metabolism.%目的 探讨成纤维细胞生长因21(FGF-21)对小鼠肝脏和脂肪细胞糖脂代谢及重要转录因子的影响.方法 分别构建FGF-21过表达及表达缺陷的小鼠肝细胞和脂肪细胞模型,测定各组细胞葡萄糖转运率、甘油三酯含量以及重要转录因子的表达改变.结果 FGF-21过表达的肝脏和脂肪细胞内FGF-21表达均显著增加(4.8倍和4.2倍,P<0.05),而表达缺陷的各组细胞FGF-21分别降低约86.3%和77.8%(P<0.05).过表达FGF-21的脂肪细胞葡萄糖转运率升高约74%,甘油三酯降低约45%,细胞内葡萄糖转运子1、胰岛素受体底物1、PPARγ、脂肪甘油三酯脂肪酶(ATGL)、激素敏感性脂肪酶(HSL)和脂肪细胞脂肪酸结合蛋白2(ap2)增加(均P<0.05);而表达缺陷时葡萄糖转运率降低约31.2%,甘油三酯升高约36.9%,除胰岛素受体底物1外其余转录因子表达均降低(均P<0.05).FGF-21对肝细胞葡萄糖转运率无显著影响(P>0.05).而FGF-21过表达的肝细胞内PPARγ、羟甲基戊二酸单酰辅酶A还原酶(HMGR)、磷酸烯醇式丙酮酸羧激酶(PEPCK)降低,低密度脂蛋白受体表达升高;表达缺陷时则相反(P<0.05).结论 FGF-21可能通过影响脂肪细胞葡萄糖摄取和肝细胞糖异生从而调节糖代谢平衡;而且
FGF-21可能在脂肪细胞脂质代谢和肝脏胆固醇代谢中具有重要作用. 【总页数】4页(699-702)
【关键词】成纤维细胞因子21;糖-脂代谢;转录因子 【作者】李钶;李伶;杨刚毅
【作者单位】400010,重庆医科大学附属第二医院内分泌科;重庆医科大学检验系临床生化教研室和教育部临床检验诊断学重点实验室;400010,重庆医科大学附属第二医院内分泌科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-endocrinology-metabolism_thesis/0201232589450.html 【相关文献】
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成纤维细胞生长因子21调控小鼠肝脏和脂肪细胞的糖脂代谢
