电针对淀粉样前体蛋白转基因小鼠海马微血管淀粉样沉积
的影响及其与低密度脂蛋白相关受体1的关系
薛卫国;张忠;许红;吴海霞;白丽敏
【期刊名称】《针刺研究》 【年(卷),期】2011(036)002
【摘要】目的:探讨电针治疗阿尔茨海默病的可能作用机制.方法:将13月龄的淀粉样前体蛋白(APP)转基因小鼠随机分为模型组和电针治疗组,以相同月龄和背景的C 57 BL/6小鼠作对照组.电针治疗组取\百会\涌泉\穴,每次留针15 min,隔日1次,针刺3个月.以Lashley Ⅲ水迷宫测定各组小鼠的学习记忆能力,以免疫组化方法检测海马B淀粉样蛋白(amyloid β protein,Aβ)、低密度脂蛋白相关受体1(low density lipoprotein receptor-related protein 1,LRP 1)的表达.结果:模型组水迷宫游出时间较对照组延长(P<0.05),海马沟微血管壁Aβ1-42表达的累积吸光度较对照组升高(P<0.01),海马沟微血管壁LRP 1表达较对照组减低(P<0.01);而电针治疗组的水迷宫游出时间明显低于模型组,海马沟微血管壁Aβ1-42表达低于模型组,海马沟微血管壁LRP 1表达高于模型组(均P<0.05).结论:电针治疗可能通过提高脑微血管壁Aβ受体LRP 1的清除转运能力,降低APP转基因鼠脑微血管壁的Aβ沉积,从而改善其学习、记忆能力.%Objective To investigate the effect of electroacupuncture (EA) of “Baihui” (GV20) and “Yongquan” (KI1) on the expression of amyloid β protein (Aβ) and low density lipoprotein (LDL) receptor-related protein 1 (LRP 1 ) in the hippocampal sulcus microvessels in amyloid precursor protein (APP) 695 V 717 Ⅰ transgenic mice, so as to study its mechanism under lying
relief of Alzheimer Disease (AD). Methods Twelve APP 695 V 717 Ⅰ transgenic mice were randomly divided into model group and EA group. Six C 57 BL/6 mice were used as the control group. EA (2 Hz/100 Hz, 3-5 mA) was applied to “Baihui”(GV 20) and “Yongquan” (KI1) for 15 min, once every other day for three months. The learning-memory ability of mice was detected by using Lashley Ⅲ water maze system. The expression level of Aβ1-42, and LRP 1 in the hippocampal sulcus microvessels were measured by immunohistochemistry. Results Water maze test showed that the swimming duration from the start to the goal box (terminal) in the Lashley Ⅲ water maze was significantly longer in the model group than in the control group (P＜0.05),suggesting a markedly lower learning-memory capacity of APP 695 V 717 Ⅰ transgenic mice. Compared with the model group, the swimming duration in the EA group was decreased considerably (P＜0.05). The integrated optical density (IOD) value of hippocampal Aβ1-42 immunoreaction (IR) positive products in the model group was significantly higher than that in the control group (P＜0.01) and the IOD value of hippocampal LRP 1 IR-positive products in the model group was apparently Io-wer than that in the control group (P＜0. 01 ). In comparison with the model group, the IOD value of Aβ1-42 iR-positive products in tie EA group was obviously lower than that in the model group (P＜0.05), while that of LRP 1 IR-positive products in the EA
group was significantly higher than that in the model group (P＜0.05), suggesting down-regulation of hippocampal Aβ1-42 expression and up-regulation of LRP 1 expression after EA, and reduction of deposition of Aβ in the cerebral microvessels after EA. Conclusion EA can improve the learning-memory capacity of APP transgenic mice, which is closely related to its effects in up-regulating hippocampal LRP 1 expression and down-regulating hippocampal Aβ1-42 expression. 【总页数】6页(95-100)
【关键词】阿尔茨海默病;电针;APP转基因鼠;海马沟;Aβ1-42;LRP1 【作者】薛卫国;张忠;许红;吴海霞;白丽敏
【作者单位】北京中医药大学基础医学院人体形态系,北京,100029;北京中医药大学基础医学院人体形态系,北京,100029;北京中医药大学基础医学院人体形态系,北京,100029;北京中医药大学基础医学院人体形态系,北京,100029;北京中医药大学基础医学院人体形态系,北京,100029 【正文语种】中文 【中图分类】R245.9+7 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_acupuncture-research_thesis/0201219113703.html 【相关文献】
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电针对淀粉样前体蛋白转基因小鼠海马微血管淀粉样沉积的影响及其
