细颗粒物对慢性阻塞性肺疾病小鼠肺组织Nrf2水平的影响
及与氧化应激的关系
赵启君;刘晓菊;曾晓丽;包海荣
【期刊名称】《中华医学杂志》 【年(卷),期】2016(096)028
【摘要】Objective To explore the effects of fine particulate matter on the level of nuclear factor erythroid-2 related factor 2 (Nrf2) in pulmonary tissues of chronic obstructive pulmonary disease (COPD)mouse models and its relationship with oxidative stress.Methods Totally 40 BALB/c mice were randomly divided into normal control group,normal PM2.5 group,COPD control group and COPD PM2.5 group.COPD mice were established using exposure of cigarette smoking.PM2.5 (20 mg/kg) was intratracheally instilled in PM2.5 group mice.Mice pulmonary function was measured by mice noninvasive body plethysmograph and lung histopathology was observed in normal control group and normal PM2.5 group mice.The mRNA and protein expression of Nrf2 was measured with real-time polymerase chain reaction (PCR) and Western blot methods.Total antioxidative capacity (TAC) was measured by O-phenanthroline colorimetry.Glutathione peroxidase (GSH-PX) was measured by improved Hafeman colorimetry and malondialdehyde (MDA) by thiobarbiturieacid colorimetry.Results Nrf2 mRNA and protein in normal control group,normal PM2.5
细颗粒物对慢性阻塞性肺疾病小鼠肺组织Nrf2水平的影响及与氧化应激的关系
