Excessive ammonia inhibited transcription of MsU2 gene and furthermore affected accumulati

Excessive ammonia inhibited transcription of MsU2

gene and furthermore affected accumulation distribution of allantoin and amino acids in alfalfa

Medicago sativa

WANG Li;JIANG Lin-lin;Nomura Mika;Tajima Shigeyuki;CHENG Xian-guo

【期刊名称】《农业科学学报（英文版）》 【年(卷),期】2015(000)007

【摘要】In legume plants, uricase gene (Nodulin-35) plays a positive role in metabolism of ureide and amide compounds in symbiotic nitrogen-ifxing in the nodules. In this study, a pot experiment was performed to examine the effects of ammonium application on the transcription of MsU2 gene and distribution of major nitrogen compounds in alfalfa Medicago sativa. Data showed that alfalfa plant has a signiifcant difference in contents of nitrogen compounds in xylem saps compared with soybean plant, and belongs to typical amide type legume plants with little ureide accumulation, and the accumulation of asparagines and ureide in the tissues of alfalfa is mainly gathered in the nodules. Northern blotting showed that excessive ammonium signiifcantly inhibited the transcription of MsU2 gene in the nodules and roots, and mRNA accumulation of MsU2 gene in the plants exposed to excessive ammonium decreased gradual y with culture time extension, indicating that application of ammonium signiifcantly

inhibited the transcription of MsU2 gene in the alfalfa plants. Although the application of exces-sive ammonium increased the contents of amino acids in various tissues of alfalfa, the accumulation of al antoin relfecting the strength of uricase activity is remarkably reduced in the xylem saps, stems and nodules when alfalfa plants exposed to excessive ammonium, suggesting that application of excessive ammonium generated a negative effect on symbiosis ifxing-nitrogen system due to inhibition of ammonium ion on uricase activity in the nodules of alfalfa. This result seems to imply that application of excessive ammonium in legume plants should not be proposed to avoid affecting the ability of ifxing nitrogen in the nodules of legume plants, and reasonable dose of ammonium should be recommended to effectively utilize the ifxed N from atmosphere in legume plant production. 【总页数】14页(1269-1282) 【关键词】

【作者】WANG Li;JIANG Lin-lin;Nomura Mika;Tajima Shigeyuki;CHENG Xian-guo

【作者单位】Col ege of Life Science, Shanxi Normal University, Linfen 041004, P.R.China;Key Laboratory of Plant Nutrition and Fertilizers, Agriculture of Ministry/Insititute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China;Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-

cho, Kita-gun, Kagawa, 761-0795, Japan;Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan;Key Laboratory of Plant Nutrition and Fertilizers, Agriculture of Ministry/Insititute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_journal-integrative-agriculture_thesis/020126185889.html 【相关文献】

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