多效蛋白基因沉默对小细胞肺癌H446细胞中部分肿瘤生
长相关基因表达的影响
余勇;施敏骅;徐迅;张增利;胡华成
【期刊名称】《中华肿瘤杂志》 【年(卷),期】2010(032)006
【摘要】Objective To investigate the changes in expression profiles of angiomotin ( Amot), schlafen5 ( Slfn5 ), metalloproteinase-9 ( MMP-9) and vascular endothelial cell growth factor ( VEGF) , which are genes associated with angiogenesis, tumor growth and invasion, after gene silencing of pleiotrophin (PTN) in human small cell lung cancer H446 cells. Methods PTN expression in H446 cells was determined by RT-PCR and Western blot. After constructing a lentiviral vector interfering PTN expression, it was packaged into virus in 293T cells. Then the virus was used to infect human small cell lung cancer H446 cells. The expressions of Amot, Slfn5, MMP-9 and VEGF were detected by RT-PCR in normal noninterference group, negative control group, PTN-interference group and group combining PTN interference and chemotherapy. Results The results of RT-PCR and Western blot test showed that PTN expression in H446 cells was high. The interference efficiency of constructed ShRNA sequences (GCAGCTGTGGATACTGCTGAA) targeting PTN was as high as 72. 1% and 59. 2% at the mRNA and protein levels, respectively, in H446 cells. Compared with the negative control group, the expressions of
多效蛋白基因沉默对小细胞肺癌H446细胞中部分肿瘤生长相关基因表达的影响
