Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mim
Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mimicking
microenvironment of tumor
Yuenan Li;Miaomiao Hai;Yu Zhao;Yalei Lv;Yi He;Guo Chen;Liyu Liu;Ruchuan Liu;Guigen Zhang
【期刊名称】《中国物理：英文版》 【年(卷),期】2018(027)012
【摘要】Development of an in vitro three-dimensional (3D) model that closely mimics actual environment of tissue has become extraordinarily important for anti-cancer study. In recent years, various 3D cell culture systems have been developed, with multicellular tumor spheroids being the most popular and effective model. In this work, we present a microfluidic device used as a robust platform for generating core–shell hydrogel microspheres with precisely controlled sizes and varied components of hydrogel matrix. To gain a better understanding of the governing mechanism of microsphere formation, computational models based on multiphase flow were developed to numerically model the droplet generation and velocity field evolution process with COMSOL Multiphysics software. Our modeling results show good agreement with experiments in size dependence on flow rate as well as effect of vortex flow on microsphere formation. With real-time tuning of the flow rates of aqueous phase and oil phase, tumor cells were encapsulated into the
Controlled generation of cell–laden hydrogel microspheres with core–shell scaffold mim
