Effect of using ascorbic acid and cysteamine supplementation onin-vitrodevelopment of buff

Effect of using ascorbic acid and cysteamine supplementation onin-vitrodevelopment of buffalo

embryos

Al-shimaa Al-H. H. El-Naby;Karima Gh.M. Mahmoud;Gamal A.M. Sosa;Mahmoud E.A. Abouel-Roos;Youssef F. Ahmed

【期刊名称】《亚太生殖杂志（英文版）》 【年(卷),期】2017(006)002

【摘要】Objective:To improvein vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).Methods:Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant

(ascorbic

acid

+

cysteamine)

or

ascorbic

acid

only.Results:There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved embryo development than ascorbic acid alone.Conclusions: Using of 50 μM L-ascorbic acid duringin vitro