Expression of Transcription Factor Oct4 in Bladder Cancer Cell Line T24 and Its Effects on
Expression of Transcription Factor Oct4 in Bladder
Cancer Cell Line T24 and Its Effects on the Biological Characteristics of the Cells
Zhiqiang ZHU;Jianguo WEN;Xiangyu ZHENG;Daoxie WANG;Qingwei WANG;Changhui FAN
【期刊名称】《华中科技大学学报(医学)(英德文版)》 【年(卷),期】2009(029)001
【摘要】The expression of octamer binding factor 4 (Oct4) gene in bladder cancer cell line T24 and its effects on the biological characteristics of the cells were investigated.RT-PCR and Western blot were employed to detect the expression of Oct4 in T24 cells.The changes of biological charac-teristics in T24 cells were analyzed before and after gene-silencing by Boyden chamber and MTT.The results showed that the expression of Oct4 gene was detectable in T24 cells by RT-PCR and Western blot.The expression of Oct4 gene and protein was down-regulated by siRNA,and average number of transwell cells in interference group,negative control group and blank control group was 101.40±4.56,104.20±10.03 and 111.00±11.90,respectively.There was significant difference in the proliferation ability of the cells from 48 h,72 h to 96 h after the interference by siRNA between in-terference group and negative group or blank control group (P<0.05).It was suggested that Oct4 gene was related with proliferation ability ofT24 cells,but not
with invasive capability. 【总页数】4页(73-76) 【关键词】
【作者】Zhiqiang ZHU;Jianguo WEN;Xiangyu ZHENG;Daoxie WANG;Qingwei WANG;Changhui FAN
【作者单位】Department of Emergency Center and Pathology,The First Hospital of Zhengzhou University,Institute of Clinical Medical Research of
Henan
Universities,Zhengzhou
450052,China;Department
of
Emergency Center and Pathology,The First Hospital of Zhengzhou University,Institute
of
Clinical
Medical
Research
of
Henan
Universities,Zhengzhou 450052,China;Department of Emergency Center and Pathology,The First Hospital of Zhengzhou University,Institute of Clinical
Medical
Research
of
Henan
Universities,Zhengzhou
450052,China;Department of Emergency Center and Pathology,The First Hospital of Zhengzhou University,Institute of Clinical Medical Research of
Henan
Universities,Zhengzhou
450052,China;Department
of
Emergency Center and Pathology,The First Hospital of Zhengzhou University,Institute
of
Clinical
Medical
Research
of
Henan
Universities,Zhengzhou 450052,China;Department of Emergency Center and Pathology,The First Hospital of Zhengzhou University,Institute of Clinical
Medical
Research
of
Henan
Universities,Zhengzhou
450052,China
【正文语种】中文 【中图分类】R5 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_journal-huazhong-university-science-technology-medical-english-german_thesis/0201245976000.html 【相关文献】
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