pET28a
Over-expression vector
Bgl II(4964)Sph I(4775)ApaL I*(4262)Mlu I(4242)Apa I(4039)EcoR V(3796)Pvu II*(3646)Xba I(5030)Nco I(5069)Nde I(5134)Tt7f1BamH I(5167)EcoR I(5173)Sac I(5183)Hind III(5192)Eco52 I(5199)Not I(5199)Xho I(5207)Drd I*(289)PT7His-taglacI(CDS)pET28a5369bpKanr(CDS)Pvu II*(3553)Pvu II*(2554)Drd I*(2454)ApaL I*(2327)Sap I(2258)ColE1Sma I(1069)Cla I(1250)ApaL I*(1827)Drd I*(2039)
Sequence around MCS
Bgl II 4921 GCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGA Xba I 4981 AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAA T7 promotor lac operator Nco I 5041 TTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCAC rbs His tag Nde I Nhe I 5101 AGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATG
BamH I EcoR I Sac I Sal I Hind III Not I Xho I 5161 GGTCGCGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCA 5221 CCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGC His tag P28T701 5281 CACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTT T7 terminator 5341 TTTGCTGAAAGGAGGAACTATATCCGGAT P28T7t
Copy number of the vector is similar to pBR322.
T7 promoter only could be recognized by T7 RNA polymerase
pET28a 图谱
