新型骨软骨支架辅助Mosaicplasty技术和携带增强基因
的ADSCs对大面积骨软骨缺损的修复效果
阮世强;邓江;徐林;黄文良;田仁元
【期刊名称】《中国现代医学杂志》 【年(卷),期】2017(027)030
【摘要】Objective To investigate the effect of new osteochondral scaffold-assisted Mosaicplasty technology and genetically enhanced adipose-derived stem cells (ADSCs) on repair of large area osteochondral defect.Methods Beagle dog model of cartilage full-thickness defect was established.The defect was filled with multiple cartilage scaffolds through Mosaicplasty technology.No fixation was performed in group E.BMP-2-ADSCs was implanted in scaffold in group D.SF/CS/nHA was implanted in scaffold in group C.SF/CS/nHA combined with BMP-2-ADSCs was implanted in scaffold in group B.SF/CS/NHA and BMP-2-ADSCs combined with PRP gel complex was implanted in scaffold in group A.Morphological changes of tissue were observed at the 4th,8th,and 16th week post operation,and tissues were harvested at time point of the 16th week post operation.Collagen Ⅰ and Collagen Ⅱ mRNA were measured by PRC.Ultimate stress,strain value and the corresponding elastic modulus of the cartilage were determined.Results Compared with group E,cartilages in group A-D experienced better healing procedure with improvement of smooth
新型骨软骨支架辅助Mosaicplasty技术和携带增强基因的ADSCs对大面积骨
