Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore

Controlled expression of enhanced green fluorescent protein and hepatitis B virus precore

protein in mammalian cells

佚名

【期刊名称】《自然科学进展（英文版）》 【年(卷),期】2003(013)002

【摘要】A novel tetracycline regulation expression system was used to regulate the expression of enhanced green fluorescent protein (EGFP) and hepatitis B virus precore protein in the mammalian cell lines with lipofectAMINE. Flow cytometry assays showed that application of the system resulted in about 18-fold induction of EGFP expression in CHO cell lines and 5-fold induction in SSMC-7721 cells and about 2-fold in the HEK293 cells. Furthermore, the effective use of this system for the controlled expression of HBV precore protein gene in hepatocellular carcinoma cells was tested. 【总页数】5页(114-118)

【关键词】tetracycline inducible expression system;enhanced green fluorescent protein;hepatitis B virus;transdominant negative proteins 【作者】佚名

【作者单位】Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China;Laboratory of Tissue Repair and Gene Transfer, Division of Plastic Surgery, Brigham and Women's Hospital, Harvard