固相萃取-高效液相色谱法测定水质中16种多环芳烃
史鑫源;王小菊;刘保献;常淼;赵红帅;沈秀娥
【期刊名称】《现代仪器与医疗》 【年(卷),期】2014(000)006
【摘要】建立固相萃取-高效液相色谱法测定水质中16种多环芳烃，研究不同色谱条件对16种多环芳烃分离效果的影响，得出最佳分离条件；并通过系统考察样品的流速、洗脱条件和有机改性剂加入量对测定的影响，得出最佳固相萃取条件。研究结果表明，利用ZORBAX ECLIPSE PAH色谱分离专用柱，以甲醇和水为流动相，采用梯度淋洗和变化的紫外、荧光程序可以很好分析16种多环芳烃，分离度均在2.0以上；采用固相萃取-高效液相色谱法测定水中16种多环芳烃，5种不同水质的加标回收率均可达60%-110%，且准确度和精密度较高，RSD均小于10%，标准曲线线性均能在0.999以上。同时，采用荧光为检测器，本方法中目标化合物的方法检出限为10-200 pg/L，可以很好满足目前监测要求。%A method of solid phase extraction and high performance liquid chromatography determination of 16 polycyclic aromatic hydrocarbons (PAHs) in water has been established. In order to acquire the best conditions, various parameters were studied, like as the deferent parameters of liquid chromatography, the sample flow rates, eluent volume and organic modifier concentration. All of the components were separated completely from each other on the special chromatographic column of ZORBAX ECLIPSE PAH with methanol-water gradient elution, and UV-detector and lfuorescence detector. In addition,
固相萃取-高效液相色谱法测定水质中16种多环芳烃
