Effect of antikeratin microinjection on the embryonic development of Xenopus laevis

Effect of antikeratin microinjection on the embryonic development of Xenopus laevis

YUHAOJIAN;JINGWUXIE;等

【期刊名称】《细胞研究：英文版》 【年(卷),期】1993(003)002

【摘要】Anti-keratin monoclonal antibody AF5 was introduced into fertilized eggs of Xenopus laevis.,and its effects on embryonic development were studied.Survival rate of the antikeratin-injected embryos was much lower(only 35.67% at gastrula)than that of the control(74.85% at gastrula),in which embryos were injected with mouse IgG.Most of survivors in the experimental series showed aberrant external appearance.On the other hand,in cleavage stage,ie 2-7h after fertilization,immunohistochemical staining of embryos showed that the expermental embryos were mostly keratin negative,while embryos of the control ones were keratin positive.When introducing this antikeratin into one cell of a 2-cell embryo,only the uninjected half of the embryo continued its development while the other half could not develop at all.These results suggested that intact keratin cytoskeleton in early embryos is indispensable to the embryonic development of Xenopus laevis.

【总页数】10页(147-156)

【关键词】爪蟾;抗角质蛋白显微注射;胚胎发育;2细胞期;单抗

【作者】YUHAOJIAN;JINGWUXIE;等 【

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DepartmentofBiology,BeijingUniversity,Beijing100871,China;DepartmentofBiology,BeijingUniversity,Beiji 【正文语种】英文 【中图分类】Q132.7 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_cell-research-english_thesis/0201268375076.html 【相关文献】

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