VitaminsCholine chlorideD-Calcium pantothenateFolic AcidNiacinamidePyridoxine hydrochlorideRiboflavinThiamine hydrochloridei-InositolInorganic SaltsCalcium Chloride (CaCl2-2H2O)Ferric Nitrate (Fe(NO3)3\Magnesium Sulfate (MgSO4-7H2O)Potassium Chloride (KCl)Sodium Bicarbonate (NaHCO3)Sodium Chloride (NaCl)Sodium Phosphate monobasic (NaH2PO4-2H2O)147.0404.0246.075.084.058.0156.0264.00.1200.0400.03700.06400.0141.01.79591832.4752476E-40.81300815.333333544.04762110.3448260.90384614140.0477.0441.0122.0206.0376.0337.0180.04.04.04.04.04.00.44.07.20.0285714290.0083857440.0090702950.0327868830.0194174760.00106382980.0118694360.04Other ComponentsD-Glucose (Dextrose)180.01000.05.5555553Phenol Red376.415.00.039851222Sodium Pyruvate110.0110.01.0Microvascular Growth Supplement (MVGS) (Gibco?)medium are: ? fetal bovine serum (4.9% v/v final concentration) ? hydrocortisone (1 μg/ml) ? human fibroblast growth factor (3 ng/ml) ? heparin (10 μg/ml) ? human epidermal growth factor (1 ng/ml) ? dibutyryl cyclic AMP (0.08 mM)
【课件-实用生物医学实验技术】_细胞分离培养与保存技巧
