脑胶质瘤相关新基因PKIβ的表达与蛋白质性质的研究
祁震宇;惠国桢;李瑶;顾少华;谢毅
【期刊名称】《中华医学杂志》 【年(卷),期】2008(088)025
【摘要】Objective To obtain differentially expressed genes related to human glioma using cDNA microarray and to characterization of one novel full-length gene. Method Four samples of human glioma samples, 1 fetal brain tissue sample, and 2 normal brain tissue samples were used to extract the total RNA,and the mRNA was used to make probes. After hybridization and washing procedure, the products of hybridization were scanned using computer system. One gene, named 436F11 clone, was subsequently analyzed by Northern blotting, bioinformatics, and protein expression. Result Fifteen differentially expressed new genes related to human glioma were obtained through four times of hybridization and scanning.Northern blotting confirmed that over-expression of 436F11 gene in the normal human brain tissue and lowexpression in the human glioma tissues. The analysis of BLASTn and BLASTx showed that the clone of 436F11 was a novel full-length gene coding 78 amino acids of protein with a theoretical relative molecular weight of 8648 and an isoelectric point of 4.69 and that it was 60% identical to mouse PKIβ amino acid, so it was called human PKIβ gene. After it was transfected into Escherichia. coli, higher-expressed protein
脑胶质瘤相关新基因PKIβ的表达与蛋白质性质的研究
