hPDGF-B基因转染对Beagle犬牙龈成纤维细胞生物学活
性的影响
钟泉;闫福华;李艳芬;陈超
【期刊名称】《福建医科大学学报》 【年(卷),期】2013(000)006
【摘要】Objective To evaluate the biological effects of human platelet-derived growth factor-B gene transfection on gingival fibroblasts . Methods The plasmid(EX-A0380-M03) carried human plate-let-derived growth factor-B(hPDGF-B) gene was transfected into gingival fibroblasts by LipofectamineTM 2000 . Morphological changes of transfected cells were recorded by optical and fluorescent microscope . Then ,MTT and flow cytometry were used to estimate the proliferation and apoptosis of gene-modified cells . The expression of collagen I and XII ,as well as theα-SMA(α-Smooth Muscle Actin) was detected by Real-time Quantitative PCR . Results Compared to the normal cells ,partial transfected cells presen-ted startlike ,polygonal ,and neuron-like . Cells proliferation was enhanced after gene interference ,but apoptosis did not appeared . At transcription level ,the expression of Collagen I was up-regulated ,while Collagen XII and α-SMA were down-regulated . Conclusion The enhanced proliferation of gingival fibro-blasts were due to paracrine and autocrine modes of fusion protein PDGF-BB-eGFP synthetized by gene modified cells . Transfeced cells did not present
hPDGF-B基因转染对Beagle犬牙龈成纤维细胞生物学活性的影响
