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在未选育组中，脾组织基因的表达量在48 h显著高于其对照组(P < 0.05)，脾组织与脾对照组基因的表达量在96~288 h并无明显差异(P > 0.05) (如图2(c))；肾组织基因的表达量在48~288 h均无明显差异(P > 0.05) (如图2(d))。
3.3. 选育组与未选育组中整合素β1基因相对表达量比较
将选育组和未选育组进行比较，在脾、肾组织及其对照组中选育组基因的表达量均低于未选育组，其中在48 h选育组和未选育组的基因表达量均达到最高。
在脾组织中，基因在192 h、288 h选育组表达量显著低于未选育组(P < 0.05)，而在240 h差异达到极显著(P < 0.01) (如图3(a))；在脾组织对照组中，基因在192 h、240 h选育组表达量显著低于未选育组(P < 0.05)，在288 h差异达到极显著(P < 0.001) (如图3(b))。
在肾组织中，基因在48 h、144 h、192 h、240 h、288 h选育组表达量相比未选育组，差异极显著(P < 0.01，P < 0.001，P < 0.01，P < 0.01，P < 0.001) (如图3(c))；在肾组织对照组中，基因在144 h、192 h选育组表达量显著低于未选育组(P < 0.05)，在240 h、288 h差异达到极显著(P < 0.001) (如图3(d))。
注：标记*不同表示组内存在显著性差异(*P < 0.05, **P < 0.01, ***P<0.001)
Figure 3. Comparison of relative expression of integrin b1 in spleen and kidney tissue at different infection stages. (a) Ex-pression of spleen tissue selection group and non-selection group; (b) Expression of spleen tissue control group and non-selection group; (c) Expression of kidney tissue selection and non-selection group; (d) Expression of the kidney tissue control group and non-selection group
图3. 对比不同感染阶段脾、肾组织中整合素β1基因相对表达情况。(a) 脾组织选育组及未选育组表达情况；(b) 脾组织对照组选育组及未选育组表达情况；(c) 肾组织选育组及未选育组表达情况；(d) 肾组织对照组选育组及未选育组表达情况
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4. 讨论
为了了解CyHV-3对鲤鱼整合素β1表达的影响，本研究测定了不同感染时间鲤鱼脾、肾整合素β1的表达。结果表明，整合素β1的相对表达在感染早期48 h最高，脾、肾组织与其对照组相比差异具有显著性(p < 0.01) (图3(a)，图3(b))。此外，还发现随着感染时间的延长，整合素β1的相对表达逐渐降低。另一方面，整合素β1的表达在感染后48 h最高，48 h、96 h、144 h显著降低。值得注意的是，我们发现整合素β1的表达水平与其在相应感染期的转录水平相似。以上结果提示，整合素β1可能在鲤鱼头肾CyHV-3感染早期起重要作用。
CyHV-3病是由Koi疱疹病毒(KHV)引起的鲤鱼(Cyprinus carpio)感染的常见病[20]，死亡率超过80% [21] [22]。CyHV-3对世界水产养殖业造成了巨大的经济损失，严重威胁着鲤鱼产业的发展。先前的报告表明，鲤鱼的免疫系统在CyHV-3感染的早期阶段起着关键作用[23]。鲤鱼头肾作为一种免疫组织[24]，本研究表明，在CyHV-3感染早期，头肾中整合素β1的表达显著增加，并随着感染时间的延长而逐渐减少，表明当鲤鱼头肾中的整合素β1在CyHV-3感染早期可能具有重要的功能被感染了。整合素介导细胞与细胞外基质的粘附，先前的研究发现整合素胞质结构域调节病毒感染性[25]。此外，还发现整合素β1在感染CyHV-3早期有差异表达。虽然需要进一步研究整合素β1在鲤鱼体内的作用，但我们的发现提示，在病毒感染早期，头肾整合素β1可能参与鲤鱼CyHV-3感染。与对照组相比，感染早期整合素β1的表达水平明显升高，提示整合素β1可能调节鲤鱼头肾CyHV-3感染，可能与CyHV-3进入鲤鱼有关。
基金项目
本研究由中央级公益性科研院所基本科研业务费专项(2018HY-ZD0205)、国家大宗淡水鱼类产业技术体系鲤种质资源与品种改良(CARS-45-06)、农业部财政专项物种资源保护(2130135)项目共同资助。
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整合素β1在鲤CyHV-3病毒中基因相对表达量研究 - 图文
