Cloning and Expression Analysis of Two Dehydrodolichyl Diphosphate Synthase Genes from Tri

Cloning and Expression Analysis of Two

Dehydrodolichyl Diphosphate Synthase Genes from

Tripterygium wilfordii

Lin-Hui Gao;Ping Su;Yi-Feng Zhang;Li-Chan Tu;Yu-Jun Zhao;Tian-Yuan Hu;Jia-Wei Zhou;Bao-Wei Ma;Wei Gao;Lu-Qi Huang;;;;;;;;;;;;;;;;;;;;;

【期刊名称】《世界中医药杂志：英文版》 【年(卷),期】2024(000)001

【摘要】Objective: To clone and investigate two dehydrodolichyl diphosphate synthase genes of Tripterygium wilfordii by bioinformatics and tissue expression analysis. Materials and Methods: According to the T. wifordii transcriptome database, specific primers were designed to clone the TwDHDDS1 and TwDHDDS2 genes via PCR. Based on the cloned sequences, protein structure prediction, multiple sequence alignment and phylogenetic tree construction were performed. The expression levels of the genes in different tissues of T. wilfordii were measured by real?time quantitative PCR. Results: The TwDHDDS1 gene encompassed a 873 bp open reading frame(ORF) and encoded a protein of 290 amino acids. The calculated molecular weight of the translated protein was about 33.46 kDa, and the theoretical isoelectric point(pI) was 8.67. The TwDHDDS2 encompassed a 768 bp ORF, encoding a protein of 255 amino acids with a calculated molecular weight of about 21.19 kDa, and a theoretical isoelectric point(pI) of 7.72.