Three transcription factors and the way immune cells affected by different plasma change i

Three transcription factors and the way immune cells affected by different plasma change in opposite ways in the development of the syndrome

of pre-eclampsia

Liang Zhou;Zhu Jing;Wang Yunfei;Wang You;Zhang Yu;Lin Jianhua;Di Wen

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2014(127)012

【摘要】Background How the transcriptional factors regulated the innate and adaptive immune system in pregnancy and preeclampsia are less understood.Nevertheless,what the plasma work in the development of this disease was not sure.The present study was design to evaluate what the transcriptional factors change in innate and adaptive immune system and what the plasma do in this filed.Methods Peripheral blood mononuclear cells (PBMC) from non-pregnant women (n=18),women with clinically normal pregnancies (n=23) and women with pre-eclampsia (n=20) were separated from peripheral blood to isolate monocytes and T cells.The purity of monocytes and T cells were analysed by flow cytometry.Monocytes and T cells were stimulated in either

lipopolysaccharides

(LPS)

or

phorbol-myristate-acetate

(PMA),respectively.Transcription Factor Arrays were used to screen the transcription factors of interest in comparing of different groups.PBMC were isolated from another 8 nonpregnant samples were co-incubated