Endothelial progenitor cell mediates transport of hepatitis B virus into myocardial tissue

Endothelial progenitor cell mediates transport of

hepatitis B virus into myocardial tissue

佚名

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2008(121)003

【摘要】Background Hepatitis B virus (HBV) replication has been reported

to

be

involved

in

many

extrahepatic

viral

disorders;however,the mechanism by which HBV is transinfected into extrahepatic tissues such as myocardium and causes HBV associated myocarditis remains largely unknown.Methods In this study,endothelial progenitor cells (EPCs) were infected by HBV and then transfused into ischemic model of mice.HBV surface and core antigen as well as mutation

of

HBV

particles

were

cell

detected sorter

by and

immunonistochemistry,fluorescent activated

transmission electron microscopy in vitro and in vivo.Results Human cord blood EPCs, but not human umbilical vein endothelial cells (HUVECs) could be effectively infected by taking up HBV in vitro.HBV envelope surface and core antigen expressions were first detectable in EPCs at day 3 after virus challenge,sustained for up to 11 days,and decreased thereafter.Similarly,the virus particles were the most abundant in EPCs ln the first week observed by a transmission electron microscope,and declined in 3 weeks after HBV infection.HBV DNA but

not HBV cccDNA in EPCs were detectable even 3 weeks after virus challenge,as

shown

by

PCR

analysis.Furthermore,intravenous

transplantation of HBV-treated EPCs into myocardial infarction Sprague & Dawley rats model resulted in incorporation of both EPCs and HBV into injured endothelial tissues of capillaries in the ischemic border zone.Conclusions These results strongly support that EPCs serve as virus carrier mediating HBV trans-infection into the injured myocardial tissues.The findings might suggest a novel mechanism for HBV-associated myocarditis. 【总页数】9页(248-256) 【

关

键

词

】

myocarditis;infection;myocardial

infarction;cord

blooc;angiogenesis 【作者】佚名

【作者单位】Department of Cardiology First Affliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;Department of

Cardiology

First

Affliated

Hospital

of

Nanjing

Medical

University,Nanjing,Jiangsu 210029,China;Department of Cardiology First Affliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;Department of Infectious Disease First Affliated Hospital of

Nanjing

Medical

University,Nanjing,Jiangsu

210029,China;Department of Hematology First Affliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;Department

of Infectious Disease First Affliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;Department of Cardiology First Affliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;Department of Cardiology First Affliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China;Department of

Cardiology

First

Affliated

Hospital

of

Nanjing

Medical

University,Nanjing,Jiangsu 210029,China 【正文语种】中文 【中图分类】R5 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-medical-journal_thesis/0201254688557.html 【相关文献】

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