慢性应激及再次应激后大鼠行为与海马tau蛋白的改变

慢性应激及再次应激后大鼠行为与海马tau蛋白的改变

杨灿;王高华;王惠玲;刘忠纯;朱志先;刘浩;郭鑫;郭俊慧

【期刊名称】《中华行为医学与脑科学杂志》 【年(卷),期】2013(022)009

【摘要】Objective To investigate behavior and hippocampal tau alterations

following

chronic

unpredictable

mild

stress

(CUMS),treatment of fluoxetine and re-exposure to CUMS.Methods 32 male SpragueDawley (SD) rats were divided into four groups,with 8 exposed to 21 consecutive days of chronic unprcdicted mild stresses (CUMS) and treated with vehicle,8 exposed to CUMS and treated with fluoxetine,8 exposed to CUMS and treated with fluoxetine and re-exposed to CUMS after washout,and 8 as normal controls treated with vehicle.Behavioral changes in these rats were analyzed.The expression of hippocampal tau and phospho-tau were analyzed using Western Blot.Results (1) Compared with the normal rats,sucrose preference ((52.37 ± 16.76) ％),total traveling distance((2736.59 ± 511.20) cm),velocity((5.69 ± 1.09) cm/s) and frequencies of rearing(2.50 ±2.00) were reduced (P ＜ 0.01) in the depressed rats.These behavioral changes could be reversed after 21d fluoxetine treatment.Compared with the control and CUMS group,sucrose preference ((42.00 ± 8.03)％),total traveling distance ((763.48 ± 457.29) cm),velocity ((2.64 ± 0.97) cm/s) and frequencies of rearing (0.25 ± 0.46)were reduced (P＜0.01 for both)