实时荧光定量PCR检测石蜡标本中麻风菌DNA
杨荣德;谭福跃;邢燕;刘键;温艳;李桓英
【期刊名称】《中国麻风皮肤病杂志》 【年(卷),期】2013(000)007
【摘要】Objective:To assess the application of real time quantitative PCR assay for the detection of M. leprae DNA in paraffin-embedded skin biopsy specimens. Methods:Primers and prob were designed accord￣ing to the repetitive sequence of M. leprae from gen Bank. The recombinant plasmid pGEMT-101 was con￣structed and served as a template to prepare the standard curve for TaqMan real time PCR. The M. Lerae DNA in paraffin-embedded skin biopsy specimens was detected and the sensitivity of the results was assessed. Re￣sults:The paraffin-embedded skin biopsy specimens from 52 leprosy patients were tested. The sensitivities of the results varied in different types of the disease:LL 100% (8/8),BL 80% (8/10),BT 78.57% (22/28) and TT 50% (3/6),respectively. Conclusion:Real-time PCR is a rapid,specific and sensitive method for the detection of M. leprae DNA in paraffin-embedded skin biopsy specimens.% 目的：评价实时定量荧光PCR(Real-time PCR)法检测石蜡标本中麻风菌DNA的应用价值。方法：根据基因库(GenBank)发表的M. leprae的全基因组序列，以一段重复序列(repetitive ele￣ment，RLEP)为扩增靶序列，合成引物和探针，构建质粒pGEMT-101作为标准品，用Real-time PCR对石蜡标本进行麻风菌DNA检
实时荧光定量PCR检测石蜡标本中麻风菌DNA
