Effects of propofol, midazolam and thiopental sodium on outcome and amino acids accumulati

Effects of propofol, midazolam and thiopental sodium on outcome and amino acids accumulation

in focal cerebral ischemia-reperfusion in rats

陈莲华;贡沁燕;肖常思

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2003(116)002

【摘要】Objective To investigate the effects of propofol, midazolam and thiopental sodium on outco mes and amino acid accumulation in focal cerebral ischemia-reperfusion in rats .Methods Male Sprague Dawley (SD) rats were scheduled to undergo 3-hour middle cerebral artery occlusion by intraluminal suture and 24-hour reperfusion. Neurologic outcomes were scored on a 0-5 grading scale. Infarct volume was shown with triphenyltetrazolium c hloride staining and measured by an image analysis system. Concentrations of va rious amino acids (aspartate, glutamate, glycine, taurine, and gama-aminobutyri c acid) were measured after 3 hours of reperfusion using high performance liquid chromatography. Propofol, midazolam and thiopental sodium were given intraperi toneally at the beginning of reperfusion.Results Both propofol and midazolam attenuated neurological deficits and reduced infarct and edema volumes. Propofol showed better neurological protection than midazol am while thiopental sodium did not exhibit any protective effect. Both propofol and midazolam