miR-200c affects the mRNA expression of E-cadherin by regulating the mRNA level of TCF8 du

miR-200c affects the mRNA expression of E-cadherin by regulating the mRNA level of TCF8 during post-natal epididymal development in

juvenile rats

Junfeng Wang;Kangcheng Ruan

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2010(042)009

【摘要】The unique temporal expression pattern of miR-200c in epididymis during post-natal development in juvenile rats was revealed by our home-made miRNA microarray in this paper.It was found that miR-200c expressed in the lowest level at Day 7 and then increased to the highest at Day 36 followed by a dramatic decrease.The pattern was exactly inverse to that of mRNA expression of transcrip tion factor 8(TCF8)revealed

by

quantitative

real-time

polymerase

chain

reaction(qRT-PCR),providing an extra evidence that TCF8 is one degradation target of miR-200c even in epididymis.Moreover,the qRT-PCR study on expression of E-cadherin and interleukin-2 indicated that miR-200c does exert an obvious effect on the mRNA expression of E-cadherin by directly regulating the mRNA level of TCF8,although the effect on interleukin-2 is not obvious as on E-cadherin,which implicates that interleukin-2 may be also regulated by other factors besides TCF8 in rat epididymis.