甲硫氨酸腺苷转移酶1A在雌雄大鼠肝脏中性别差异表达研
究
韦垒;何跃明;庄冰洁;刘权焰;刘志苏;袁玉峰
【期刊名称】《中华实验外科杂志》 【年(卷),期】2015(032)005
【摘要】Objective To observe the changes of Sprague-Dawley rat sex hormone levels influence the expression of the methionine adenosyltransferase-1A (MAT1 A) in rats liver tissues.Methods To select 36 sexually mature Sprague-Dawley rats,build a SD rat model of sex hormone concentration gradient difference (male and female have three groups,each group of 6).Using enzyme linked immunosorbent assay (ELISA) method to measure the sex hormone concentrations of each group rats and the expressions of MAT1A were examined by the Western blotting and real-time quantitative polymerase chain reaction (RT-qPCR).Results ELISA' s results:Estrogen concentrations of FM0 group is (43.125 ±11.066) ng/L,FM + group is 0 ng/L,FM + T group is (31.738 ± 7.142) ng/L (P ＜ 0.01);Androgen concentrations of M0 group is (28.945 ± 9.379) mmol/L,M + group is 0 mmol/L,M + E group is (26.158 ±7.846) mmol/L (P ＜0.01).RT-qPCR:females MAT1A expression is FM0 ＞ FM + ＞ FM +T,with a mean of 0.471 4 ＞0.133 4 ＞0.066 0 (P ＜0.01),males MAT1A expression is M0 ＜ M + ＜ M + E,with an average of 0.029 8 ＜ 0.068 7 ＜ 0.205 5 (P ＜ 0.01).Western blotting:Male and
甲硫氨酸腺苷转移酶1A在雌雄大鼠肝脏中性别差异表达研究
