Supplementary data
Characterisation of algal organic matter produced by bloom-forming marine and freshwater algae
L.O. Villacortea,b,*, Y. Ekowatia, T.R. Neuc, J.M. Kleijnd, H. Winterse,f, G. Amya,e,g, J.C. Schippersa and M.D. Kennedy a,g
a
UNESCO-IHE Institute for Water Education, Westvest 7, 2611 AX Delft, Netherlands
Wetsus Center of Excellence for Sustainable Water Technology, Agora 1, 8934 CJ Leeuwarden, Netherlands
b
c
Department of River Ecology, Helmholtz Centre for Environmental Research (UFZ), Brueckstrasse 3A, 39114 Magdeburg, Germany
d
Laboratory of Physical Chemistry and Colloid Science, Wageningen University, Dreijenplein 6, 6703HB Wageningen, Netherlands
e
Water Desalination and Reuse Center, 4700 KAUST, Thuwal, Saudi Arabia
f
Fairleigh Dickinson University, Teaneck, New Jersey, 07666, USA Delft University of Technology, Stevinweg 1, 2628 CN Delft, Netherlands
g
* Corresponding author. Current address: FMC Technologies, Delta 101, 6825 MN Arnhem, Netherlands; Tel.: +31267999137; E-mail address: loreen.villacorte@gmail.com
1
Supplementary data S1 - Composition of artificial seawater and algal media Table S1-1: Artificial seawater (ASW) composition.
Chemical ion Chlorine, Cl Sodium, Na Sulfate, SO4 Magnesium, Mg Calcium, Ca Potassium, K
Hydrogen Carbonate HCO Carbonate, CO3 Bromine, Br Total
-
2-
3-
+2+
2+ 2- +-Concentration (g/L)
19.2 10.7 2.7 1.3 0.42 0.39 0.16 0.001 0.05 34.3
Table S1-2: L1 medium for marine dinoflagellates (www.ccap.ac.uk)
Stocks
(1) Trace elements (from CCMP) FeCl3·6H2O Na2EDTA·2H2O CuSO4·5H2O Na2MoO4·2H2O ZnSO4·7H2O CoCl2·6H2O MnCl2·4H2O H2SeO3 NiSO4·6H2O Na3VO4 K2CrO4 (2) Vitamin mix
Cyanocobalamin (Vitamin B12) Thiamine HCl (Vitamin B1) Biotin Medium NaNo3 NaH2PO4.2H2O
Trace elements stock solution (1) Vitamin mix stock solution (2)
3.15 g 4.36 g 0.25 mL 3 mL 1 mL 1 mL 1 mL 1 mL 1 mL 1 mL 1 mL 0.0005 g 0.1 g 0.0005 g per liter 0.075 g 0.00565 g 1. 0 ml 1. 0 ml
- - -
- - 2.45 19.9 22 10 180 1.3 2.7 1.84 1.94
per liter
stock solution (g/L)
2
Table S1-3: Guillard’s medium (f/2 + Si) for marine diatoms (www.ccap.ac.uk).
Stocks
(1) Trace elements (chelated) NA2EDTA FeCl3.6H2O CuSO4.5H2O ZnSO4.7H2O CoCl2.6H2O MnCl2.4H2O Na2Mo4.2H2O (2) Vitamin mix
Cyanocobalamin (Vitamin B12) Thiamine HCl (Vitamin B1) Biotin
(3) Sodium metasilicate Na2SiO3.9H2O Medium NaNO3 NaH2PO4.2H2O
Trace elements stock solution (1) Vitamin mix stock solution (2) Sodium metasilicate stock solution (3)
per litre 4.16 g 3.15 g 0.01 g 0.022 g 0.01 g 0.18 g 0.006 g 0.0005 g 0.1 g 0.0005 g 30.0g 0.075 g 0.00565 g 1.0 ml 1.0 ml 1.0 ml
Table S1-4: BG11 medium for freshwater blue-green algae and protozoa (www.ccap.ac.uk).
Stocks (1) NaNO3 (2) K2HPO4 (3) MgSO4.7H2O (4) CaCl2.2H2O (5) Citric acid
(6) Ammonium ferric citrate green (7) Na2 EDTA (8) Na2CO3
(9) Trace metal solution: H3BO3 MnCl2.4H2O ZnSO4.7H2O Na2MoO4.2H2O CuSO4.5H2O Co(NO3)2.6H2O Medium Stock solution 1 Stock solutions 2 - 8 Stock solution 9
2.86 g 1.81 g 0.22 g 0.39 g 0.08 g 0.05 g per litre 100.0 ml 10.0 ml each 1 ml per litre 15.0 g 4.0 g 7.5 g 3.6 g 0.6 g 0.6 g 0.1 g 2 g
3
mmc
