丙戊酸对胶质瘤细胞株T98-G体外作用实验研究
邵翠杰;陈芙蓉;关皑丽;陈忠平
【期刊名称】《中华神经外科杂志》 【年(卷),期】2009(025)002
【摘要】Objective To investigate the anti-tumor activity of 2-propylpentanoic acid (VPA), a common anti-epilepsy drug at clinical safety dosage, and the effect on the expression of Acetyl-Histone H3 and H4 expression. Methods Human glioma cell line T98-G was treated by VPA at concentration of 0. 2, 0. 6,1.0,2.0,2.7,3.6,4. 5 mmol/L for 1,2,3,5 and 7 days, respectively. The Cellular proliferation was determined by the methyl thiazolyl tetrazolimn (MTT) assay, and cell cycle arrest and cell apoptosis were observed by flow cytometry. The expression of Acetyl-Histone H3 and H4 were detected by Western Blotting analysis. Results Obvious changes in cell proliferation was found in the glioma cell line T98-G after treatment with VPA in respective concentration for 24 h, and in a dose-and time-dependent manner. G1 Or G2/M phase arrest was induced by the clinical safety (dosage) concentration 1.0 mmol/L at 24 hours. Cells in S phase were decreased about 10% while cells in G1、G2/M phase were increased accordingly. The expression of Aceyl-Histone H3 and H4 were significantly increased after treatment with 1.0 mmol/L VPA at 24 hours, and in a time-dependent manner. Conclusion VPA may inhibit growth of
丙戊酸对胶质瘤细胞株T98-G体外作用实验研究
