刚地弓形虫RH株SAG3蛋白抗体检测的ELISA方法建立
马亮;任保彦;左绍志;宫鹏涛;李建华;张国才;杨举;张西臣
【期刊名称】《安徽农业科学》 【年(卷),期】2012(040)015
【摘要】[ Objective] The research aimed to establish the indirect ELISA for detecting the purified surface protein SAG3 antibody of Toxo-plama gondii(T. gondii) . [Method] Using chessboard titration method, the best conditions of indirect ELISA were determined. Taking negative serum as control,the positive serums of mouse against Neospora caninum, G. lamblia, C. andersoni, E. tendla and C. parvum were used to detect if the indirect ELISA has cross reactions with them. [ Result ] The optimal coating conditions were as follows: antigen coating concentration was 0. 25 (μg/hole, and the optimal dilution of the detected serum of T. gondii and enzyme-labeled second antibody were 1:400 and 1:3 000 respectively, blocking agent was the PBST solution of 5% nonfat-dry milk. The developed indirect ELISA had no cross reaction with positive serums. The sensitivity of this method was 1: 1 600. [ Conclusion] This method had high sentivity,stronger specificity and better repeatability , which might be used for the diagnosis and epidemiological investigation of T. gondii with SAG3 protein.%[目的]利用纯化的刚地弓形虫RH株表面蛋白SAG3的原核重组蛋白为基础,建立抗体间接ELISA检测方法.[方法]采用棋盘滴定法,确定间接ELISA的最佳条件.采用鼠抗新孢子虫阳性血
刚地弓形虫RH株SAG3蛋白抗体检测的ELISA方法建立
