Er：YAG 激光照射对人牙周膜成纤维细胞增殖的影响
周志雄;张笋
【期刊名称】《实用口腔医学杂志》 【年(卷),期】2016(032)003
【摘要】目的：观察离体培养的人牙周膜成纤维细胞在 Er：YAG 激光照射后其增殖能力的变化。方法：离体培养的人牙周膜成纤维细胞，进行常规免疫组化染色，鉴定细胞来源。选用第5代人牙周膜成纤维细胞，随机分为5组，A 组为对照组，不接受激光照射。实验组分别接受频率为10 Hz 的不同能量 Er：YAG 激光照射，参数分别为 B 组：50 mJ，C 组：100 mJ，D 组：150 mJ，E 组：200 mJ。分别于1、3、5、7、9 d 使用 CCK-8法测定细胞生长情况，分析其增殖能力的变化。结果：培养第5天时 B～E 组细胞增殖率高于 A 组（P ＜0．05）。结论：低能量，短时间的 Er：YAG 激光照射能促进牙周膜成纤维细胞的增殖能力。%Objective:To observe the effect of Er:YAG laser on the proliferation of human periodontal ligament fibroblasts(hP-DLFs).Methods:Human periodontal ligament fibroblasts were cultured in vitro and identified by immunohistochemistry.The cells of 5th passage were divided into 5 groups.The cells in group A without treatment were used as the controls,in group B,C,D and E were treated with Er:YAG laser of 10 Hz at 50 mJ,100 mJ,150 mJ and E-200 mJ for 1 s respectively.The proliferation of the cells was examined on day 1,3,5,7,9 by CCK-8.Results:The proliferation of hPDLFs in group B,C,D and E increased more than that in group A(P <0.05)5 d after Er:YAG laser
Er：YAG 激光照射对人牙周膜成纤维细胞增殖的影响
