五味护肝咀嚼片质量标准研究
王会娟;毛春芹;陆兔林;王菊;顾娟娟;吴爽爽
【期刊名称】《南京中医药大学学报》 【年(卷),期】2013(029)001
【摘要】OBJECTIVE To establish quality standard of Wuwei Hugan Chewable Tablets. METHODS With chisantherin B, Deoxyschisandrin and Schisandra as control materials, Wuwei Hugan Chewable Tablet is identified by thin layer chromatog-raphy (TLC); the content of schisandrin, schisantherin B, schisantherin A, Deoxyschisandrin and Schisandrin B, are simultaneously determinated by HPLC. RESULTS Excellent chromatographic separation was achieved in the five tested lignans with good linearity (r>0.999 5) over the studied concentration ranges. The average extraction recovery (i?SD)(n = 9) was 99.90% (1.63%) for Schisandrin, 99.42%(1. 72%) for Schisandrol B, 99. 81 %(2. 07%) for Schisantherin A, 99. 79%(1. 91%) for Deoxyschisandrin and 100.57%(1.61%) for Schisandrin B.CONCLUSION Specific, sensitive and reliable TLC identification and HPLC content determination of Wuwei Hugan Chewable Tablets were established. The TLC and HPLC methods developed for simultaneous determination of five lignans are simple and valid. It can be used for comprehensively controlling quality of Wuwei Hugan Chewable Tablets.%目的 建立五味护肝咀嚼片的质量标准.方法 采用薄层色谱法以五味子醇乙、五味子甲素和五味子对照药材为对照,对制剂
五味护肝咀嚼片质量标准研究
